An ELISA kit is a type of diagnostic test kit used to detect and quantify the presence of specific proteins or other molecules in a biological sample, such as blood or urine. ELISA stands for Enzyme-Linked Immunosorbent Assay, which describes the method by which the test works.
In an ELISA test, a sample is added to a microplate well that has been coated with a specific antibody or antigen. If the target molecule is present in the sample, it will bind to the antibody or antigen on the plate. Then, a second antibody is added that recognizes a different part of the target molecule and is linked to an enzyme. When a substrate is added, the enzyme catalyzes a reaction that produces a detectable signal, typically a color change.
ELISA kits are widely used in medical and biological research as well as in clinical diagnostic laboratories. They can be used to detect a variety of molecules, including hormones, viral or bacterial antigens, and antibodies produced by the immune system.
BTL Biotechnolabs is an authorized ELISA Kit Distributor in India.
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